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Clinical Chemistry 18: 73-75, 1972;
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Clinical Chemistry, Vol 18, 73-75, Copyright © 1972 by the American Association for Clinical Chemistry

Clinical Nephelometry: 1. New, Accurate, Heat-Precipitation Nephelometric Method for Rapid Determination of Plasma Fibrinogen

Eugene W. Rice 1 and D. E. R. Muesse 1

1 Pathology Department, Allentown Hospital Association, Allentown, Pa. 18102 (E.W.R.), and Hach Diagnostics, P.O. Box 907, Ames, Iowa 50010 (D.E.R.M.).

We have developed an accurate procedure for measurement of plasma fibrinogen. Turbidity resulting from heating (56°C, 20 min) plasma diluted with a new "zwitterionic" buffered-saline reagent (pH 5.6) is nephelometrically measured (600 nm). We evaluated the method both by confirming the accuracy of its prestandardization and calibration on a new clinical nephelometer, and by comparative standardization by use of a conventional spectrophotometer. The nephelometer is more sensitive and gives a linear response over a wide range of concentrations. Representative information supporting the broad clinical utility of the determination of plasma fibrinogen is illustrated. These assays should be a significant complement to muititest biochemical screening of both healthy and hospitalized individuals.


Key Words: diagnostic aids • spectrophotometry and nephelometry compared • stress evaluation • multitest screening • buffer as diluent

Submitted on July 24, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.