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Clinical Chemistry 18: 77-79, 1972;
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Clinical Chemistry, Vol 18, 77-79, Copyright © 1972 by the American Association for Clinical Chemistry

Plasma Calcium Assay, with Use of the Jellyfish Protein, Aequorin, as a Reagent

K. T. Izutsu 1 and S. P. Felton 1

1 Anesthesia Research Center, Department of Anesthesiology, University of Washington, School of Medicine, Seattle, Wash. 98105.

A previously proposed assay for plasma calcium, suitable for routine clinical measurements, has been investigated. The method is based on the calcium-sensitive luminescence of a jellyfish protein, aequorin. Advantages of this method are low cost, simplicity, and the suitability of most currently available fluorometers and spectrophotometers for the assay. Disadvantages are that aequorin may not be readily available, and that the assay apparently yields only the total and (with suitable sample preparation) the diffusible calcium concentrations.


Key Words: fluorometry of calcium • total and diffusible (ultrafiltrable, "dialyzable") calcium

Submitted on August 4, 1971




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