Clinical Chemistry
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Clinical Chemistry 18: 1318-1322, 1972;
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Clinical Chemistry, Vol 18, 1318-1322, Copyright © 1972 by the American Association for Clinical Chemistry

Automated Procedure for Assay of 2,3-Diphosphoglycerate in Red Cells by Measuring Enzymatically Released Inorganic Phosphorus

James B. Powell 1, Clarence E. Emery 1, and Gaylon A. Peyton 1

1 Division of Biochemistry, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20012.

An automated technique is reported for measuring erythrocyte 2,3-diphosphoglycerate (DPG). We have automated a specific method, in which the DPG phosphatase activity of phosphoglycerate mutase is used and potentiated by phosphoglycolate. Precision, accuracy, recovery, and comparability with a reference method are discussed.


Key Words: phosphoglycerate mutase • P[unknown] measurement after release • normal values • hypoxia • colorimetry • blood banking • AutoAnalyzer • phosphoglycolate

Submitted on May 10, 1972
Accepted on July 14, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.