Clinical Chemistry
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Clinical Chemistry 18: 1323-1325, 1972;
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Clinical Chemistry, Vol 18, 1323-1325, Copyright © 1972 by the American Association for Clinical Chemistry

Kinetic Nephelometric Procedure for Measurement of Amylase Activity in Serum

James R. Shipe 1 and John Savory 1

1 Departments of Medicine, Biochemistry, and Pathology, University of North Carolina, Chapel Hill, N.C. 27514; and the Department of Pathology, University of Florida, Gainesville, Fla. 32601.

We developed a kinetic procedure for determination of amylase activity in serum by use of nephelometric measurements. Light scattering from the substrate, a stable suspension of starch, is decreased as amylase hydrolyzes the starch to soluble fragments. Values obtained for serum amylase correlate closely with those determined by a method in which a starch-dye complex is used. Precision of the new procedure is 3.4% (relative standard deviation). Units of amylase activity can either be expressed in terms of milligrams of starch consumed or converted to more conventional amylase units.


Key Words: light-scattering measurements • liquid starch substrate

Submitted on May 30, 1972
Accepted on July 17, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.