Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 18: 1329-1333, 1972;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Monforte, J.
Right arrow Articles by Sunshine, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Monforte, J.
Right arrow Articles by Sunshine, I.

Clinical Chemistry, Vol 18, 1329-1333, Copyright © 1972 by the American Association for Clinical Chemistry

Fluorometric Determination of Primary and Secondary Amines in Blood and Urine after Thin-Layer Chromatography

Joseph Monforte 1, Raymond J. Bath 1, and Irving Sunshine 1

1 Cuyahoga County Coroner's Office, 2121 Adelbert Rd., Cleveland, Ohio 44106; and the Department of Pathology, School of Medicine Case Western Reserve University, Cleveland, Ohio 44106.

7-Chloro-4-nitrobenzo-2-oxa -1,3-diazole reacts with primary and secondary amines to produce highly fluorescent compounds. The resulting emission spectra for primary and secondary amines are similar; however, when the excitation spectra of these compounds are determined when the emission wavelength is kept at 520 nm, distinctly different results are obtained. Thin-layer chromatography of suitably concentrated aliquots of these solutions yield characteristic spots, the Rf values of which can be used to help identify the substances involved. This reaction was applied to the quantitative determination of amphetamine in biological extracts. If amphetamine is the only drug present it can be quantitated by fluorometry and confirmed by the thin-layer chromatographic procedure. If more than one drug is present, the fluorometric procedure is unsatisfactory for quantitative analysis. Thin-layer chromatography can be useful to identify qualitatively the drugs involved. Gas chromatography is the procedure of choice in these situations.


Key Words: amphetamine • spectrophotofluorometry • toxicology • drug abuse • thin-layer chromatography • gas chromatography

Submitted on May 17, 1972
Accepted on July 24, 1972






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1972 by the American Association for Clinical Chemistry.