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Clinical Chemistry 18: 1339-1342, 1972;
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Clinical Chemistry, Vol 18, 1339-1342, Copyright © 1972 by the American Association for Clinical Chemistry

Radioimmunoassay of Serum Renin Activity and Digoxin Concentrations, with Use of Polyethylene Glycol to Separate Free and Antibody-Bound Ligand

M. James Barrett 1 and Patricia S. Cohen 1

1 J. L. Goforth Laboratory, Inc., Dallas, Texas 75201.

Polyethylene glycol in sodium barbital buffer (25 mmol/liter, pH 8.6) was used to separate free from antibody-bound ligand in the radioimmunoassay of serum digoxin and renin. Polyethylene glycol (final concentration, 150 g/kg of buffer) gave maximum sensitivity in these assays. The length of time between addition of polyethylene glycol and centrifugation of precipitated antibody does not affect sensitivity.


Key Words: angiotensin I • charcoal in separation of free and antibody-bound ligand

Submitted on May 23, 1972
Accepted on July 24, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.