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Clinical Chemistry 18: 1343-1349, 1972;
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Clinical Chemistry, Vol 18, 1343-1349, Copyright © 1972 by the American Association for Clinical Chemistry

Gas-Liquid Chromatographic Isolation, Identification, and Quantitation of Some Barbiturates, Glutethimide, and Diphenylhydantoin in Whole Blood

Rodney G. Cooper 1, Mavis S. Greaves 1, and Goronwy Owen 1

1 Rheumatism Research Unit, Nether Edge Hospital, Sheffield S11 9EL, and Chemical Pathology Department, Northern General Hospital, Sheffield S5 7AU, U.K.

An accurate method is described for the qualitative and quantitative determination of 11 barbiturates, glutethimide, and diphenylhydantoin in a single extract obtained from 1 ml of whole blood, which is extracted with ether at pH 2 and separately but simultaneously gas-liquid chromatographed on a selective (3% OV-17) and a nonselective (5% OV-1) column. Retention data are given. Results for barbiturate obtained by the Broughton [Biochem. J. 63, 207 (1956)] spectrophotometric method and this gas-liquid chromatographic method are compared. Blood from more than 30 patients has been analyzed, and data are given to illustrate some of the findings.


Key Words: primaclone (primadone) • 5-allyl-neopentylbarbituric acid • phenylmethylbarbituric acid • emergency procedures

Submitted on May 15, 1972
Accepted on July 26, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.