Clinical Chemistry
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Clinical Chemistry 18: 1350-1354, 1972;
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Clinical Chemistry, Vol 18, 1350-1354, Copyright © 1972 by the American Association for Clinical Chemistry

Use of 3-Methyl-2-Benzothiazolone Hydrazone in an Automated Colorimetric Method for Determination of Triglycerides in Plasma or Serum

W. E. Neeley 1, G. E. Goldman 1, and C. A. Cupas 1

1 Institute of Pathology and Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106.

A new automated colorimetric method is described for the determination of serum or plasma triglycerides. The samples are extracted with 2-propanol containing activated alumina. An AutoAnalyzer Sampler II and Proportioning Pump II are used in combination with a Gilford Model 300 N spectrophotometer and Model 3091-A debubbler flow cell. Following on-line saponification, glycerol is oxidatively cleaved with sodium metaperiodate. The resulting formaldehyde is reacted with 3-methyl-2-benzothiazolone hydrazone (MBTH) under mild conditions to produce the corresponding azine. Further reaction of MBTH with the azine in the presence of an oxidizing agent results in the formation of a blue cationic adduct. Beer's law is obeyed, at 670 nm, to a triglyceride concentration of 400 mg/100 ml. Because of the high sensitivity of the MBTH reaction, short incubation periods, high sampling rates (60/h), and low flow rates can be used. The results correlate well with those obtained by the Kessler-Lederer method and by a totally enzymatic method.


Key Words: rases AutoAnalyzer • hyperlipoproteinemia • normal range

Submitted on June 19, 1972
Accepted on July 27, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.