Clinical Chemistry
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Clinical Chemistry 18: 1355-1359, 1972;
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Clinical Chemistry, Vol 18, 1355-1359, Copyright © 1972 by the American Association for Clinical Chemistry

Interlaboratory and Intralaboratory Comparison of T3-Uptake Methodology

F. W. Spierto 1, Ivey Lois Hubert 1, and Robert F. Witter 1

1 Center for Disease Control, Health Services and Mental Health Administration, U.S. Department of HEW, Atlanta, Ga. 30333.

Triiodothyronine (T3)-uptake values in selected human serum pools were compared by use of Abbott's "Trisorb," Mallinckrodt Nuclear's "Res-O-Mat," Curtis Nuclear's "Tri-lonex," and a standard procedure. In addition, interlaboratory variation was measured among laboratories using the Abbott and Mallinckrodt procedures. Study of methodological variables indicated that incubation time and temperature affect T3 measurement. The Abbott procedure is most liable to variations caused by either time or temperature changes; the Mallinckrodt and Curtis methods are least liable to variations caused by changes in time and temperature, respectively. Between-run variation for sera with low T3-uptake values was greater than within-run variation as measured by the Abbott, Mallinckrodt, or standard procedure. For sera with high T3-uptake values, between-run variation and within-run variation are similar; with the Curtis method this was true for all serum pools. We and the reference laboratories found the Abbott method to be most sensitive in detecting changes in T3-uptake values of samples in the "hyperthyroid" range.


Key Words: resin triiodothyronine uptake • T3, kit methods • hypo- and hyperthyroidism, diagnosis • simulated hypo- and hyperthyroid serum • thyroid status

Submitted on May 5, 1972
Accepted on July 28, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.