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Clinical Chemistry 18: 1373-1375, 1972;
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Clinical Chemistry, Vol 18, 1373-1375, Copyright © 1972 by the American Association for Clinical Chemistry

Serum Propylthiouracil: Determination by a Direct Colorimetric Procedure

Charles R. Ratliff 1, Paul F. Gilliland 1, and Frank F. Hall 1

1 Sections of Biochemistry and Endocrinology, Scott and White Clinic, Temple, Tex. 76501.

A direct colorimetric method is described for the determination of propylthiouracil in serum, which is based on reaction of the drug at pH 8.0 with 2,6-dichloroquinone-chloroimide to produce a colored chloroform-soluble compound. After isolation from the aqueous layer with phase-separating filter paper, the chloroform solution is read against a chloroform blank at 435 nm. Propylthiouracil added to drug-free serum serves as a control sample. The method obeys Beer's law up to a concentration of at least 10 mg/liter.


Key Words: 2,6-dichloroquinone • chloroimide • thyrotoxicosis

Submitted on June 9, 1972
Accepted on August 2, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.