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Clinical Chemistry 18: 1395-1402, 1972;
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Clinical Chemistry, Vol 18, 1395-1402, Copyright © 1972 by the American Association for Clinical Chemistry

Quantitation of Plasma Aldosterone by Radioimmunoassay

M. J. St. Cyr 1, J. M. Sancho 1, and J. C. Melby 1

1 Section of Endocrinology and Metabolism, Evans Memorial Department of Clinical Research, University Hospital, Boston University School of Medicine, Boston University Medical Center, Boston, Mass. 02118.

Aldosterone antibodies were produced by injecting sheep with the albumin conjugate of aldosterone-18,21-dihemisuccinate. Conditions optimal for an accurate, reproducible assay with this antiserum were determined experimentally. Aldosterone is extracted from plasma and isolated by a single thin-layer chromatographic system before the immunoassay is performed. Normal plasma aldosterone in supine subjects is 8.6 ± 3.8 ng/100 ml; after 2 h in the upright position, 18.3 ± 9.5 ng/100 ml. Eighteen months of experience with the method have proved it to be reliable with respect to reproducibility, accuracy, and precision.


Key Words: thin-layer chromatography • free and bound steroid • normal values

Submitted on August 10, 1972
Accepted on August 18, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.