Clinical Chemistry
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Clinical Chemistry 18: 1411-1414, 1972;
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Clinical Chemistry, Vol 18, 1411-1414, Copyright © 1972 by the American Association for Clinical Chemistry

Investigation of the Use of Calcein in the Ultramicro Fluorometric Determination of Calcium in Serum

James F. Bandrowski 1 and Carl L. Benson 1

1 Becton, Dickinson and Co., UNOPETTE Engineering Department, Rutherford, N.J. 07070.

Calcium in serum is commonly determined fluorometrically with calcium-calcein as the fluorescent complex. A method is presented for analyzing calcein to determine the optimal concentration for both greatest linearity and greatest reproducibility. Serum calcium as determined with the "UNOPETTE" system is compared with results obtained by use of a Technicon SMA 12/60.

Submitted on March 18, 1971
Accepted on August 18, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.