Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 18: 1416-1419, 1972;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yee, H. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yee, H. Y.

Clinical Chemistry, Vol 18, 1416-1419, Copyright © 1972 by the American Association for Clinical Chemistry

Automated Hexokinase Procedure for Assaying Glucose in Urine, Serum, or Plasma

Hugh Y. Yee 1

1 Department of Pathology, Hutzel Hospital, 432 E. Hancock Ave., Detroit, Mich. 48201.

A colorimetric hexokinase method is described for measuring glucose in urine with the AutoAnalyzer. Pretreatment of the urine is not necessary. Sampling rate is 50/h, sample requirement 0.13 ml. Absorbance is measured at 505 nm; Beer's law is followed to 300mg/liter. Results obtained with this procedure and by one in which o-toluidine was used in combination with glucose oxidase were highly correlated (r = 0.998). Glucose concentrations in serum or plasma may be determined by diluting the sample 10-fold. Bilirubin in concentrations up to 200 mg/liter do not significantly interfere. With a dilutor, 20 µl of plasma or serum is diluted to 0.2 ml directly from a capillary collection tube after centrifugation. Thus, a convenient, sensitive method is available for assaying glucose concentrations in pediatric specimens.


Key Words: pediatric specimens AutoAnalyzer

Submitted on March 15, 1972
Accepted on July 17, 1972






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1972 by the American Association for Clinical Chemistry.