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Clinical Chemistry 18: 1419-1422, 1972;
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Clinical Chemistry, Vol 18, 1419-1422, Copyright © 1972 by the American Association for Clinical Chemistry

Simple, Rapid, Kinetic Method for Serum Creatinine Measurement

Jack A. Lustgarten 1 and Robert E. Wenk 1

1 Section of Clinical Chemistry, Division of Clinical Pathology, Department of Pathology, Sinai Hospital of Baltimore, Inc., Belvedere at Greenspring, Baltimore, Md. 21215.

A study of the reaction between creatinine and alkaline picrate confirmed that there is a linear relation between absorbance change and creatinine concentration over the entire clinical range of values. The specificity and accuracy of the method are comparable to that of the end-point Jaffé reaction with deproteinization. The test is somewhat imprecise in the low range, but the simple, rapid procedure is appropriate for the monitoring of patients with glomerular disease and those undergoing therapeutic dialysis.


Key Words: hemodialysis • emergency procedure • Jaffé reaction • urinary creatinine • renal disease • creatinine clearance • alkaline picrate reagent • uremia

Submitted on June 7, 1972
Accepted on July 22, 1972




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Copyright © 1972 by the American Association for Clinical Chemistry.