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Clinical Chemistry 18: 1425-1427, 1972;
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Clinical Chemistry, Vol 18, 1425-1427, Copyright © 1972 by the American Association for Clinical Chemistry

Spot Applicator for Quantitative Thin-Layer and Paper Chromatography, Suitable for Clinical Analysis

Arthur F. Rosenthal 1 and Marta G. Vargas 1

1 Department of Laboratories, The Long Island Jewish-Hillside Medical Center, New Hyde Park, N.Y. 11040.

Construction and operation of a semiautomatic TLC sample-spotting applicator are described. With the apparatus, multiple samples, each as large as 2.0 ml, can be transferred to a thin-layer chromatographic plate with little or no operator attention. Replicate analyses show a relative standard deviation of 1.35-1.99% without washout; liquid holdup is no more than 3-5%, and is of no significance in the analytical accuracy. The apparatus has been found to be very suitable for quantitative (as well as qualitative) thin-layer chromatographic analysis in the clinical laboratory.


Key Words: multiple-sample application • evaporative sample spotter for TLC

Submitted on June 26, 1972
Accepted on August 7, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.