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Clinical Chemistry 18: 1468-1474, 1972;
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Clinical Chemistry, Vol 18, 1468-1474, Copyright © 1972 by the American Association for Clinical Chemistry

Serum Alkaline Phosphatase: Total Activity and Isoenzyme Determinations Made by Use of the Centrifugal Fast Analyzer

Bernard E. Statland 1, H. Harold Nishi 1, and D. S. Young 1

1 Clinical Chemistry Service, Clinical Pathology Department, National Institutes of Health, Bethesda, Md. 20014.

We present a kinetic method in which the centrifugal analyzer is used to determine total alkaline phosphatase activity and to measure the isoenzymes in bone, liver, and the L-phenylalanine-sensitive fraction (intestine, tumor, and placenta). Measurements were made at 30°C in diethanolamine buffer, with p-nitrophenylphosphate as substrate. The alkaline phosphatase isoenzymes were separated and measured by selective chemical inhibition with 10 mmol/liter L-phenylalanine and 3.3 mol urea per liter. Analyses were performed on sera of a group of healthy pediatric and young adult volunteers and, in addition, on sera from patients with clinically documented osteoblastic disorders and hepatobiliary diseases. The precision and accuracy of the centrifugal analyzer were evaluated, as was the suitability of the instrument for routine clinical laboratory use.


Key Words: diagnostic aid • normal values • tissue sources of isoenzymes • pipetting error • disodium p-nitrophenyl phosphate in diethanolamine buffer as substrate • differentiation of isoenzymes • bone disease, liver disease, lung carcinoma

Submitted on July 10, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.