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Clinical Chemistry 18: 1493-1497, 1972;
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Clinical Chemistry, Vol 18, 1493-1497, Copyright © 1972 by the American Association for Clinical Chemistry

Isolation and Measurement of Pancreatic Amylase in Human Serum and Urine

L. Fridhandler 1, J. Edward Berk 1, and M. Ueda 1

1 Department of Medicine, University of California, Irvine College of Medicine, Irvine, Calif. 92668.

We describe a sensitive quantitative procedure for separating isoamylases in human serum, urine, and tissue homogenates. Two components have been discerned with chromatographic characteristics resembling those of pancreatic and salivary amylases, respectively. Several lines of evidence—derived from studies in normal subjects, pancreatectomized patients, and patients with acute pancreatitis—indicate that the pancreas is probably the source of the component in serum and urine that exhibits characteristics of pancreatic amylase. The source of the component resembling salivary amylase has not yet been fully defined. Isoamylase analysis of extracts of fallopian tube and liver revealed two amylase components with chromatographic properties similar to pancreatic and salivary amylases, respectively.


Key Words: salivary amylase • column chromatography • tissue of origin for amylases • isoenzymes • pancreatic disease • normal values • clearance of amylase • activity of tissue homogenates • diagnostic tool

Submitted on August 30, 1972




The following articles in journals at HighWire Press have cited this article:


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J. E. BERK, L. FRIDHANDLER, and R. L. NESS
Amylase and lsoamylase Activities in Renal Insufficiency
Ann Intern Med, March 1, 1979; 90(3): 351 - 353.
[Abstract] [PDF]

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M. B. BLOCK, J. E. BERK, L. S. FRIDHANDLER, D. F. STEINER, and A. H. RUBENSTEIN
Diabetic Ketoacidosis Associated with Mumps Virus Infection: Occurrence in a Patient with Macroamylasemia
Ann Intern Med, May 1, 1973; 78(5): 663 - 667.
[Abstract] [PDF]

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 ANN INTERN MEDHome page
R. W. AMMANN, J. E. BERK, L. FRIDHANDLER, M. UEDA, and W. WEGMANN
Hyperamylasemia with Carcinoma of the Lung
Ann Intern Med, April 1, 1973; 78(4): 521 - 525.
[Abstract] [PDF]


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Copyright © 1972 by the American Association for Clinical Chemistry.