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Clinical Chemistry 18: 1529-1531, 1972;
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Clinical Chemistry, Vol 18, 1529-1531, Copyright © 1972 by the American Association for Clinical Chemistry

Immunochemical Quantitation of Prostatic Phosphatase

Vita Milisauskas and 1 and Noel R. Rose 1

1 Center for Immunology and the Department of Microbiology, School of Medicine, State University of New York at Buffalo, Buffalo, N.Y. 14214.

A monkey antiserum to a fraction of human urine precipitated by concentrations of ammonium sulfate between 1.5 and 2.5 mol/liter was shown to contain antibodies specific for prostatic acid phosphatase. Using a modification of Laurell’s immunodiffusion method with a phosphatase stain, we devised a quantitative immunoassay for this enzyme.


Key Words: Laurell’s "rocket" immunodiffusion method • Ouchterlony double-diffusion technique diagnostic aid: prostatic carcinoma • enzyme measurement specificity






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Copyright © 1972 by the American Association for Clinical Chemistry.