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Clinical Chemistry 18: 1534-1536, 1972;
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Clinical Chemistry, Vol 18, 1534-1536, Copyright © 1972 by the American Association for Clinical Chemistry

Simple Method for Determination of Urinary dgr-Aminolevulinic Acid as an Index of Lead Exposure

Katsumaro Tomokuni 1 and Masana Ogata 1

1 Department of Public Health, Okayama University Medical School, 2-5-1 Shikata, Okayama, Japan.

A simple method is described for the quantitative analysis of urinary dgr-aminolevulinic acid. 2-Methyl3-carbethoxy-4-(3-propionic acid) pyrrole, produced by the condensation of dgr-aminolevulinic acid with ethyl acetoacetate, is almost completely extracted from an aqueous solution with ethyl acetate without resorting to ion-exchange column chromatography. The pyrrole is determined colorimetrically by treating an aliquot of the extract with a modified Ehrlich’s reagent.


Key Words: ethylacetoacetate condensation with dgr-aminolevulinic acid • normal values • porphobilinogen • colorimetry • environmental hazard • toxicology of lead

Submitted on July 31, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.