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Clinical Chemistry 18: 1541-1542, 1972;
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Clinical Chemistry, Vol 18, 1541-1542, Copyright © 1972 by the American Association for Clinical Chemistry

Estimation of Serum Proteins by Electrophoresis on Cellulose Acetate

J. W. Keyser 1 and G. L. Watkins 1

1 Department of Chemical Pathology, The Welsh National School of Medicine, Royal Infirmary, Cardiff CF2 1SZ, U.K.

We have evaluated results for albumin obtained by a standard procedure for cellulose acetate electrophoresis of proteins in serum. The Ponceau S-stained albumin and globulins were eluted and the albumin was calculated by the generally accepted formula [(albumin-bound dye absorbance/absorbance of total protein-bound dye) x total serum protein concn] and by the formula (absorbance of albumin-bound dye in test/absorbance of albumin-bound dye in a reference serum) x concn of albumin in reference serum. The ratio of values by the first and second methods ranged from 0.93 to 1.30, the first giving the higher results in cases of discrepancy. These findings confirm the limitations in accurately calculating any serum-protein fraction by the first method. The second method appears to be the more accurate.


Key Words: serum albumin • dye binding by proteins • calculation of results of electrophoresis

Submitted on March 6, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.