Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 18: 105-109, 1972;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Garry, P. J.
Right arrow Articles by Lubin, A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garry, P. J.
Right arrow Articles by Lubin, A. H.

Clinical Chemistry, Vol 18, 105-109, Copyright © 1972 by the American Association for Clinical Chemistry

Identification of Serum Cholinesterase Fluoride Variants by Differential Inhibition in Tris and Phosphate Buffers

Philip J. Garry 1, George M. Owen 1, and A. Harold Lubin 1

1 Department of Pediatrics, College of Medicine, The Ohio State University and Children’s Hospital Research Foundation, 561 South 17th St., Columbus, Ohio 43205.

We describe a useful technique to identify "fluoride-resistant" serum cholinesterase variants (E1uE1f and E1fE1f) by using the method of Garry to detect "atypical" variants and to measure serum cholinesterase activity [Clin. Chem. 17, 192 (1971)]. With butyrylthiocholine as substrate, sodium fluoride is used as a differential inhibitor in both Tris and phosphate buffer systems (50 mmol/liter, pH 7.4, 25°C). Fluoride inhibition values are plotted on two-dimensional graph paper, Tris plus sodium fluoride vs. phosphate plus sodium fluoride. Inhibition values for the "fluoride-resistant" and "atypical" variants are located in specific quadrants, which permits unequivocal identification of each variant. Examination of 836 Caucasian preschool children revealed a gene frequency of 0.0066 for the "fluoride" variant. No "fluoride-resistant" variants were found in 168 Negro preschool children examined.


Key Words: "fluoride" and "dibucaine" numbers • proposed mechanism of fluoride effect • fluoride resistant variant:gene frequency • racial differences in cholinesterase variants

Submitted on August 28, 1971






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1972 by the American Association for Clinical Chemistry.