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Clinical Chemistry 18: 110-115, 1972;
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Clinical Chemistry, Vol 18, 110-115, Copyright © 1972 by the American Association for Clinical Chemistry

A Study of Various Electrophoretic and Inhibition Techniques for Separating Serum Alkaline Phosphatase Isoenzymes

Roy B. Johnson Jr. 1, Karen Ellingboe 1, and Phyllis Gibbs 1

1 Scripps Clinic and Research Foundation, 476 Prospect St., La Jolla, Calif. 92037.

Optimal conditions for the identification and semiquantitation of the isoenzymes of serum alkaline phosphatase are reviewed and a new technique is presented. In the recommended scheme, specific disc electrophoretic conditions are used to produce good separations, which are improved by comparing the alkaline phosphatase patterns of sera before and after treatment with heat or urea (3 mol/liter). This method is compared with other electrophoretic and inhibition techniques. Interpretation is aided by (a) reference to the relative mobilities of each isozyme, and (b) inactivation of bone alkaline phosphatase by heat or urea. The isozymes originating from bile, intestine, placenta, bone, and liver can be independently resolved as separate bands with untreated sera except when bone alkaline phosphatase is grossly increased. The proposed method permits identification and semiquantitation of all the recognized alkaline phosphatase isozymes in most sera, as an aid to diagnosis.


Key Words: disc electrophoresis • heat and urea denaturation • diagnostic aid • isozymes from bone, liver, bile, intestine, placenta






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Copyright © 1972 by the American Association for Clinical Chemistry.