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Clinical Chemistry 18: 145-149, 1972;
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Clinical Chemistry, Vol 18, 145-149, Copyright © 1972 by the American Association for Clinical Chemistry

Rapid Large-Scale Screening of Blood Proteins by Use of Cellulose Acetate Electrophoresis on a Solid Support

Samuel T. Nerenberg 1, Linda DeMarco 1, and Carolyn Ganger 1

1 Department of Pathology, University of Illinois, Chicago, Ill. 60612.

We describe a new principle of cellulose acetate electrophoresis (solid-support electrophoresis), in which cellulose acetate membranes are given a solid support, instead of being suspended as heretofore. The new technique is particularly well suited for screening procedures, because many samples can be processed economically on a single run. Also described for the first time, in conjunction with solid-support electrophoresis, is a method of (a) simultaneously using continuous and discontinuous buffer systems with specially (series) oriented membranes, or (b) a single-sample application for fractionating different constituents simultaneously (proteins, lipoproteins, isoenzymes, etc.).


Key Words: screening procedure • multiple-constituent determination in a single run

Accepted on October 26, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.