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Clinical Chemistry 18: 188-192, 1972;
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Clinical Chemistry, Vol 18, 188-192, Copyright © 1972 by the American Association for Clinical Chemistry

Blood Carboxyhemoglobin: Results with Conventional Standards Compared with Those with a Submicroliter Reference of Gaseous CO

Gunnar Sundström 1

1 Department of Clinical Chemistry, Central Hospital, S-351 01 Växjö, Sweden.

The reference standard for the determination of CO in blood usually consists of whole blood or hemoglobin (Hb) solutions equilibrated with 100% CO. A new, simple method for injecting very small gas volumes with high accuracy and reproducibility makes possible a closer study of the CO extraction process from blood (e.g., into neutral ferricyanide). The present method for CO analysis is based on a "gas chromatographic" system with CO detection as methane by flame ionization. In preparing blood COHb standards, notice must be taken of physically dissolved CO and "COrefractory" hemoglobin (e.g., methemoglobin). Solutions to this problem are suggested. The validity of the factor 1.39 (ml) for maximal CO binding capacity of 1 g of Hb is discussed in comparison with the experimentally measured recovery, about 97% for a fully saturated Hb solution.


Key Words: CO binding capacity of Hb • methemoglobin • ferricyanide • tonometry • flame ionization • gas chromatography

Submitted on August 9, 1971
Accepted on November 1, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.