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Clinical Chemistry 18: 193-198, 1972;
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Clinical Chemistry, Vol 18, 193-198, Copyright © 1972 by the American Association for Clinical Chemistry

Automated Fluorometric Procedure for Measurement of Creatine Phosphokinase Activity

J. A. S. Rokos 1, S. B. Rosalki 1, and D. Tarlow 1

1 St. Mary’s Hospital, London W.2., England.

An automated procedure is described for measuring creatine phosphokinase activity (in serum, although the technique is adaptable) with the Technicon AutoAnalyzer. Creatine, formed by the action of the enzyme on phosphocreatine and adenosine diphosphate, is measured by the fluorescence of its reaction product with alkaline ninhydrin solution. Thirty determinations can be made per hour, and only 0.2 ml of sample is required.


Key Words: AutoAnalyzer • alkaline ninhydrin and guanidyl compounds, reaction • ultraviolet manual method, results compared • normal values for serum

Accepted on November 1, 1971







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Copyright © 1972 by the American Association for Clinical Chemistry.