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Clinical Chemistry 18: 203-205, 1972;
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Clinical Chemistry, Vol 18, 203-205, Copyright © 1972 by the American Association for Clinical Chemistry

Sensitive, Rapid Quantitation of Serum and Urinary Protein by Electroimmunodiffusion

M. C. B. de Pizzolato 1, G. B. Del Campo 1, M. A. Pizzolato 1, and C. Vergani 1

1 Istituto di Patologia Speciale Medica II, University of Milan, Via F. Sforza, 35, Milan, Italy.

A simple, rapid method is described for quantitative estimation of albumin, transferrin, and agr2-macroglobulin on strips of antibody-spread gelatinized cellulose acetate. Samples are applied on a strip with 1-µl micropipets and a volumetric distributor is used to coat the strip with 2µl of a diluted antiserum per square centimeter. Electrophoresis requires 2 h for albumin and at least 3 h for agr2-macroglobulin and transferrin. Precipitate peaks are sharply defined. As little as 10 ng of protein can be estimated, so urinary proteins can be studied without concentrating the sample.


Key Words: albumin • agr2-macroglobulin, transferrin • nanogram samples

Submitted on October 26, 1971
Accepted on November 8, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.