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Clinical Chemistry 18: 206-208, 1972;
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Clinical Chemistry, Vol 18, 206-208, Copyright © 1972 by the American Association for Clinical Chemistry

Tris/Tris·HCl: A Standard Buffer for Use in the Physiologic pH Range

Richard A. Durst 1 and Bert R. Staples 1

1 Analytical Chemistry Division, National Bureau of Standards, Washington, D.C. 20234.

A buffer solution containing tris(hydroxymethyl)-aminomethane (0.01667 mol/kg) and its hydrochloride salt (0.0500 mol/kg) is proposed as a pH standard for the physiologically important pH range of 7.3 to 7.5. Standard pH values were assigned to this reference solution at temperatures from 0° to 50°C by means of measurements with hydrogen/silver chloride cells without transference. At 37°C, the assigned pH of this buffer solution is 7.382, with a temperature coefficient of -0.026 pH unit per degree Celsius. This new standard is more compatible with biologic fluids than is the previously certified phosphate buffer.

Submitted on October 26, 1971
Accepted on November 12, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.