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Clinical Chemistry 18: 217-221, 1972;
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Clinical Chemistry, Vol 18, 217-221, Copyright © 1972 by the American Association for Clinical Chemistry

Quantitation of Beta Lipoprotein (LDL) Cholesterol by Densitometric Evaluation of Disc Electropherograms

Robert F. Moran 1, William P. Castelli 1, and Marianne V. Moran 1

1 Bon Secours Hospital, Methuen, Mass. 01844, and the Framingham Heart Study, Framingham Union Hospital, Framingham, Mass. 01701.

This paper describes a rapid, facile, and accurate new procedure for determination of marginal Type IV or Type II hyperlipoproteinemias, in which discontinuous (disc) electrophoresis on polyacrylamide gel is used to quantitate LDL (beta) cholesterol without ultracentrifugation. Fasting sera, previously assayed by ultracentrifugation and chemical technique, were prestained with Sudan Black, and the lipoproteins were separated by disc electrophoresis. The beta-lipoprotein fraction of each electrophoretic pattern was quantitated by densitometry. The values for the integrated densitometric peak areas for the beta fraction and the chemically assayed cholesterol of the ultracentrifuged LDL fraction correlated well (r = .90). Results were determined from a curve relating chemical concentration of the integrated peak values by densitometry to the known LDL cholesterol of a series of standards.


Key Words: Types II and IV hyperlipoproteinemias • densitometry • polyacrylamide gel

Accepted on November 19, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.