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Clinical Chemistry 18: 237-238, 1972;
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Clinical Chemistry, Vol 18, 237-238, Copyright © 1972 by the American Association for Clinical Chemistry

Single Radial Immunodiffusion of Undiluted Serum Proteins

M. C. B. Pizzolato 1, M. A. Pizzolato 1, and A. Agostoni 1

1 Instituto di Patologia Speciale Medica II, Univ. of Milan, Via F. Sforza, 35, Milan, Italy.

A simple method is described for quantitative determination of IgG, IgA, IgM, agr2M, and other proteins without dilution of the sample. Strips of gelatinized cellulose acetate spread with antiserum are used as a supporting medium. The method is accurate, highly reproducible, and seems particularly suitable for routine work because it permits determination of hypo-, normal, and hyperglobulinemia on a single strip.

Submitted on October 11, 1971
Accepted on November 22, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.