Clinical Chemistry
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Clinical Chemistry 18: 239-243, 1972;
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Clinical Chemistry, Vol 18, 239-243, Copyright © 1972 by the American Association for Clinical Chemistry

Automated Direct Method for Measurement of Serum Cholesterol, with Use of Primary Standards and a Stable Reagent

William R. Holub 1 and Frank A. Galli 1

1 Research and Development Department of Central Health Labotatories, 636 Wantagh Ave., Levittown, N.Y. 11756.

A manual direct determination of serum cholesterol [Clin. Chem. 16, 980 (1970)] has been adapted for automation. Optimal reaction temperature and reaction time were determined. The aqueous standard used is equivalent to NBS Standard Reference Material No. 911. Commercial control sera and 60 randomly chosen specimens were used to check accuracy and precision against the method of Abell et al. [J. Biol. Chem. 195, 357 (1952)]. The correlation coefficient was 0.980. When these results were compared with those obtained with the SMA 12/60, the latter method had significant differences when there was interference from hemoglobin, bilirubin, gamma globulin, or gross lipemia. For a pooled serum, the inter-run relative standard deviation (coefficient of variation) was ±2.7%. Advantages over other automated direct methods for serum cholesterol include: use of primary standards, the stable and less noxious reagent, simplicity of the manifold, longer life of the reagent manifold tubing, and the decrease in photometric interferences.


Key Words: AutoAnalyzer • ferric perchlorate—ethyl acetate reagent

Submitted on August 9, 1971
Accepted on November 22, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.