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Clinical Chemistry 18: 258-262, 1972;
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Clinical Chemistry, Vol 18, 258-262, Copyright © 1972 by the American Association for Clinical Chemistry

Separation and Identification of Neutral and Acidic Metabolites in Cerebrospinal Fluid

L. D. Waterbury 1 and L. A. Pearce 1

1 Departments of Pharmacology and Neurology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, N. C. 27103.

We describe a method for obtaining profiles of neutral and acidic substances present in human cerebrospinal fluid (CSF) by gas—liquid chromatography. Metabolites extracted with ethyl acetate and ether are converted to methyl ester, trimethylsilyl ether derivatives. With this technique, acidic metabolites of brain amines, and neutral metabolites of dopamine and norepinephrine have been identified in human CSF. Some of these substances have not been reported previously in CSF. Extracted substances are identified on the basis of their methylene-unit values and mass spectral data. Potential use of this method in neurodiagnosis and in delineating drug-induced changes in CSF metabolites is discussed.


Key Words: gas-liquid chromatography— mass spectrometry • brain amines • diagnosis of neural disease • methylene-unit values

Submitted on November 10, 1971
Accepted on December 3, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.