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Clinical Chemistry 18: 270-274, 1972;
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Clinical Chemistry, Vol 18, 270-274, Copyright © 1972 by the American Association for Clinical Chemistry

Specificity of Accepted Procedures for Urine Creatinine

Sheshadri Narayanan 1 and Harold D. Appleton 1

1 Department of Clinical Chemistry, Metropolitan Hospital Center and Department of Biochemistry, New York Medical College, New York, N.Y. 10029.

Specificities of various commonly used procedures for creatinine in urine were evaluated after subjecting pure creatinine or urine (with or without added creatinine) to counter-current distribution (CCD) between acetate buffer (pH 4.6) and n-butanol. After 10 transfers, each phase of each tube was analyzed for creatinine by each of the various procedures, and the resulting data were compared with the theoretical distribution curve for pure creatinine. The data indicate that the original or automated Jaffé reaction is the most nearly specific of the procedures investigated. Thus, the CCD technique, besides being a powerful separatory tool, also permits a physicochemical approach to the evaluation of specificities of standard procedures in clinical chemistry.


Key Words: counter-current distribution • Jaffé reaction • Sakaguchi reaction

Accepted on December 13, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.