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Clinical Chemistry 18: 290-292, 1972;
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Clinical Chemistry, Vol 18, 290-292, Copyright © 1972 by the American Association for Clinical Chemistry

Rapid Extraction of Triglycerides from Human Adipose Tissue with Petroleum Ether

H. A. I. Newman 1, Elizabeth A. Gordon 1, Darrol W. Heggen 1, and Martin D. Keller 1

1 Departments of Pathology and Preventive Medicine, The Ohio State University Hospitals, 410 W. 10th Ave., Columbus, Ohio 43210.

A rapid method has been developed for extracting neutral lipid from small samples of human adipose tissue, with a petroleum ether ("Skellysolve B") as the extracting solvent. It is coupled with a simple method of transesterification, to convert the purified triglycerides to fatty acid methyl esters. Virtually no phospholipid is extracted by the petroleum ether. Analysis of the extracted lipid by gas—liquid chromatography yielded fatty acid distributions comparable to those obtained by chloroform—methanol extraction.


Key Words: fatty acids • gas—liquid chromatography • phospholipids • chloroform— methanol extraction • methyl esterification

Submitted on October 12, 1971
Accepted on December 20, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.