Clinical Chemistry
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Clinical Chemistry 18: 296-298, 1972;
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Clinical Chemistry, Vol 18, 296-298, Copyright © 1972 by the American Association for Clinical Chemistry

Determination of Total Urinary Protein by Combined Gel Filtration and Automated Biuret Reaction

Karl Doetsch 1

1 Department of Clinical Chemistry, VA Hospital, Durham, N.C. 27700.

Gel filtration was used to remove non-protein-bound substances that interfere with measurement of urinary protein by an automated version of the biuret reaction. Nearly 98% of the protein was accounted for, and accuracy and precision were acceptable. Interference from bile pigments or hemoglobin pigments can be eliminated, and specificity is increased.


Key Words: AutoAnalyzer • interference removal

Accepted on November 4, 1971






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Copyright © 1972 by the American Association for Clinical Chemistry.