Clinical Chemistry
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Clinical Chemistry 18: 335-339, 1972;
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Clinical Chemistry, Vol 18, 335-339, Copyright © 1972 by the American Association for Clinical Chemistry

Manual Nephelometric Methods for Immunochemical Determination of Immunoglobulins IgG, IgA, and IgM in Human Serum

L. M. Killingsworth 1 and John Savory 1

1 Department of Pathology, College of Medicine, University of Florida; and the Veterans Administration Hospital, Gainesville, Fla. 32601.

Manual procedures are presented for determining immunoglobulins IgG, IgA, and IgM in human serum. The three immunoglobulins are quantitated, after reaction with specific antisera, by measuring light scattering by the antigen-antibody complexes. The procedure is standardized by use of pooled human serum with known concentrations of IgG, IgA, and IgM. Results with the present system correlated excellently with results of either radial immunodiffusion or an automated immunochemical procedure. Precision of duplicate determinations for the IgG, IgA, and IgM methods is 5.37, 1.30, and 5.36% (CV), respectively.


Key Words: light-scattering as function of time and wavelength • radial immunodiffusion

Submitted on November 5, 1971
Accepted on January 3, 1972




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Copyright © 1972 by the American Association for Clinical Chemistry.