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Clinical Chemistry 18: 349-351, 1972;
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Clinical Chemistry, Vol 18, 349-351, Copyright © 1972 by the American Association for Clinical Chemistry

Direct Colorimetric Determination of Hippuric Acid in Urine

Katsumaro Tomokuni 1 and Masana Ogata 1

1 Department of Public Health, Okayama University Medical School, Okayama, Japan.

A direct colorimetric method is described for determination of hippuric acid in urine. Hippuric acid dissolved in pyridine—water (1:1) produces a color (Amax = 410 nm) when benzenesulfonyl chloride is added. With the method based on this color reaction, urinary hippuric acid follows the Beer-Lambert law up to 1 mg/ml.


Key Words: benzenesulfonyl chloride reagent • assessment of exposure to toluene fumes

Submitted on December 10, 1971
Accepted on January 20, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.