Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 18: 391-392, 1972;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cantor, F.
Right arrow Articles by Fishman, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cantor, F.
Right arrow Articles by Fishman, W. H.

Clinical Chemistry, Vol 18, 391-392, Copyright © 1972 by the American Association for Clinical Chemistry

Quality Control of an Automated Differential Isoenzyme Assay of Alkaline Phosphatase, with Use of L-Phenylalanine Inhibition

Fred Cantor 1, Sidney Green 1, Leo L. Stolbach 1, and William H. Fishman 1

1 Department of Pathology (Oncology), Tufts University School of Medicine, the Cancer Research Department, New England Medical Center Hospitals; and the Enzymology Laboratory, Lemuel Shattuck Hospital, Boston, Mass. 02130.

A quality-control program, in which a heat-stable human alkaline phosphatase preparation is used to monitor the performance of an automated alkaline phosphatase method, was observed for a year. In the method phenyl phosphate is used as substrate, L-phenylalanine as an intestinal isoenzyme inhibitor, and a phenol standard curve to monitor chromogen formation. Mean total alkaline phosphatase activity was 14.0 ± 0.9 King-Armstrong units (CV, 6.4%), and mean inhibition by L-phenylalanine was 78.2 ± 1.1% (CV, 1.4%). These data compared closely with the data obtained in a collaborating laboratory during the same one-year period.


Key Words: phenyl phosphate substrate • AutoAnalyzer • placental alkaline phosphatase

Submitted on November 3, 1971
Accepted on December 13, 1971






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1972 by the American Association for Clinical Chemistry.