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Clinical Chemistry 18: 417-421, 1972;
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Clinical Chemistry, Vol 18, 417-421, Copyright © 1972 by the American Association for Clinical Chemistry

Cellulose Acetate Electrophoresis of Alkaline Phosphatase Isoenzymes in Human Serum and Tissue

H. A. Fritsche Jr. 1 and H. R. Adams-Park 1

1 Clinical Chemistry Section, Department of Clinical Pathology, The University of Texas at Houston, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025.

We describe a new electrophoretic method for the characterization of human serum and tissue alkaline phosphatases on cellulose acetate plates. Enzymes are localized fluorometrically with the substrate agr-naphthol AS-MX phosphate or colorimetrically by coupling the reaction product with Fast Blue RR. Both localization techniques are sensitive enough to demonstrate isoenzyme patterns in micro-scale samples of normal sera. Our electrophoretic studies indicate that sera of children and adults normally contain isoenzymes originating from both liver and bone. The high sensitivity of the method allows the use of normal sera as markers rather than tissue extracts, and isoenzyme patterns may be visually assessed after heat inactivation and chemical inhibition. The method is suitable for the electrophoretic fractionation of alkaline phosphatase in large numbers of sera, with equipment and technique familiar to many laboratories.


Key Words: differential diagnosis of liver and bone disease • fluorometric and colorimetric localization of enzymes on electropherograms • serum enzyme as marker • disc electrophoresis

Submitted on December 22, 1971
Accepted on February 18, 1972




The following articles in journals at HighWire Press have cited this article:


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 CLIN PEDIATRHome page
V. Abbassi, A.R. Colon, and R. H. Schwartz
Benign Elevation of Serum Alkaline Phosphatase, Transient and Persistent Variety
Clinical Pediatrics, June 1, 1984; 23(6): 336 - 337.
[Abstract] [PDF]


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Copyright © 1972 by the American Association for Clinical Chemistry.