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Clinical Chemistry 18: 427-432, 1972;
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Clinical Chemistry, Vol 18, 427-432, Copyright © 1972 by the American Association for Clinical Chemistry

Successive Determination of Na+, Cl-, and H+ Activities on the Skin Surface with Ion-Selective Electrodes

Marvin Green 1, Hans Behrendt 1, and Gloria Libien 1

1 Department of Pediatrics, New York Medical College, Metropolitan Hospital Center, New York, N.Y. 10029.

A method is described for the rapid sequential measurement of Na+, Cl-, and H+ activities on the skin surface with ion-selective electrodes. Rationales are given for details of technique such as temperature control, use of a conducting bridge, pH-dependence of sodium electrode performance, and the uniform electrode setting for all tests. Data on reproducibility document the precision of the method when applied to measurements on the skin surface. Reasons are given why Na+ and Cl- activity measurements on the skin require a different design and interpretation than skin pH determinations.


Key Words: mucoviscidosis • dry and sweating skin • normal values

Submitted on December 20, 1971
Accepted on February 23, 1972







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Copyright © 1972 by the American Association for Clinical Chemistry.