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Clinical Chemistry, Vol 18, 449-453, Copyright © 1972 by the American Association for Clinical Chemistry
1 General Clinical Research Center for Children,
Wayne State University School of Medicine, and Children’s Hospital of Michigan, Detroit, Mich. 48201.
A procedure is presented for proline quantification in serum filtrates and urine hydrolysates. Interference from primary amino acids is greatly reduced by nitrozation. After nitrozation and neutralization, proline is reacted with ninhydrin in a concentrated electrolyte solution. The resulting red proline—ninhydrin product is salted out, extracted into benzene, and measured at 520 nm. The extent and nature of interference from various amino acids have been determined, as has the effect of urinary constituents. Procedural variables have also been studied. Preliminary data are presented for urinary proline/creatinine ratios for 6-to 12-month-old infants.
Submitted on February 21, 1972
Accepted on March 7, 1972
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