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Clinical Chemistry 18: 454-458, 1972;
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Clinical Chemistry, Vol 18, 454-458, Copyright © 1972 by the American Association for Clinical Chemistry

Spectrophotometric Micromethod for Measuring Cholinesterase Activity in Serum or Plasma

Tetsuo Uete 1, Yoko Miyamato 1, Mieko Ohnishi 1, and Noriko Shimano 1

1 Kitano Hospital, Tazuke Kofukai Medical Research Institute, 3, Nishiogimachi, Kita-ku, Osaka City, Japan.

A simple ultraviolet method has been developed for determining serum or plasma cholinesterase activity. The cholinesterase acts on acetyl- or butyrylthiocholine to release thiocholine, which reacts with 2,2'- or 4,4'-dithiodipyridine, showing absorbance at 324 or 343 nm, respectively. The activity can be measured kinetically and also after incubation for 3 to 5 min at 20° to 37°C. A volume of 10 µl of serum or plasma is sufficient for the determination. This method is well suited for clinical investigation as well as experimental studies.


Key Words: release of thiocholine from acetyl- or butyryl-choline • kinetic measurement • normal values

Submitted on January 13, 1972
Accepted on March 8, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.