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Clinical Chemistry 18: 473-475, 1972;
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Clinical Chemistry, Vol 18, 473-475, Copyright © 1972 by the American Association for Clinical Chemistry

Activation of Serum Creatine Kinase by Dithiothreitol

William A. Warren 1

1 Mary Imogene Bassett Hospital, Cooperstown, N. Y. 13326.

I studied activation of serum creatine kinase (CK) by sulfhydryl reagents, and the optimum assay conditions. Dithiothreitol and beta-mercaptoethanol were the best activators. Dithioerythritol, L-cysteine, glutathione, and mercaptoacetate were less effective. Over a dithiothreitol concentration range from 0.02 to 2 mmol/liter, enzyme activity increased with increasing dithiothreitol. Activation was maximum between 2 and 20 mmol/liter. At any dithiothreitol concentration the rate of activation was rapid, and the activity attained did not increase on prolonged preincubation. Thus, the extent of activation of serum CK by dithiothreitol is largely a thermodynamically-controlled, not a kinetically-controlled, phenomenon.


Key Words: Cleland’s reagent • -SH activators of CK • spectrophotometry • myocardial and skeletal muscle injury • normal range

Submitted on January 17, 1972
Accepted on February 2, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.