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Clinical Chemistry 18: 499-502, 1972;
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Clinical Chemistry, Vol 18, 499-502, Copyright © 1972 by the American Association for Clinical Chemistry

Estimation of the Concentration of Low-Density Lipoprotein Cholesterol in Plasma, Without Use of the Preparative Ultracentrifuge

William T. Friedewald 1, Robert I. Levy 1, and Donald S. Fredrickson 1

1 Biometrics Research Branch (W.T.F.) and the Molecular Disease Branch (R.I.L. and D.S.F.), National Heart and Lung Institute, 9000 Rockville Pike, Bethesda, Md. 20014.

A method for estimating the cholesterol content of the serum low-density lipoprotein fraction (Sf0-20) is presented. The method involves measurements of fasting plasma total cholesterol, triglyceride, and high-density lipoprotein cholesterol concentrations, none of which requires the use of the preparative ultracentrifuge. Comparison of this suggested procedure with the more direct procedure, in which the ultracentrifuge is used, yielded correlation coefficients of .94 to .99, depending on the patient population compared.


Key Words: hyperlipoproteinemia classification • determination of plasma total cholesterol, triglyceride, high-density lipoprotein cholesterol • beta lipoproteins

Submitted on February 29, 1972
Accepted on March 13, 1972




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Occup Med (Lond)Home page
R. Donovan, T. Nelson, J. Peel, T. Lipsey, W. Voyles, and R. G. Israel
Cardiorespiratory fitness and the metabolic syndrome in firefighters
Occup. Med., October 1, 2009; 59(7): 487 - 492.
[Abstract] [Full Text] [PDF]


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Diabetes CareHome page
X. Yang, W. Y. So, R. C.W. Ma, G. T.C. Ko, A. P.S. Kong, H. Zhao, A. O.Y. Luk, C. W.K. Lam, C. S. Ho, P. C.Y. Tong, et al.
Low LDL Cholesterol, Albuminuria, and Statins for the Risk of Cancer in Type 2 Diabetes: The Hong Kong Diabetes Registry
Diabetes Care, October 1, 2009; 32(10): 1826 - 1832.
[Abstract] [Full Text] [PDF]


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J Am Coll CardiolHome page
J. H. Ix, R. Katz, I. H. De Boer, B. R. Kestenbaum, M. A. Allison, D. S. Siscovick, A. B. Newman, M. J. Sarnak, M. G. Shlipak, and M. H. Criqui
Association of chronic kidney disease with the spectrum of ankle brachial index the CHS (Cardiovascular Health Study).
J. Am. Coll. Cardiol., September 22, 2009; 54(13): 1176 - 1184.
[Abstract] [Full Text] [PDF]


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J. Lipid Res.Home page
G. Reyes-Soffer, S. Holleran, W. Karmally, C. I. Ngai, N.-T. Chen, M. Torres, R. Ramakrishnan, W. S. Blaner, L. Berglund, H. N. Ginsberg, et al.
Measures of postprandial lipoproteins are not associated with coronary artery disease in patients with type 2 diabetes mellitus
J. Lipid Res., September 1, 2009; 50(9): 1901 - 1909.
[Abstract] [Full Text] [PDF]


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J. Lipid Res.Home page
N. R. Matthan, M. Pencina, J. M. LaRocque, P. F. Jacques, R. B. D'Agostino, E. J. Schaefer, and A. H. Lichtenstein
Alterations in cholesterol absorption/synthesis markers characterize Framingham Offspring Study participants with CHD
J. Lipid Res., September 1, 2009; 50(9): 1927 - 1935.
[Abstract] [Full Text] [PDF]


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J. Appl. Physiol.Home page
J. S. Wooten, K. D. Biggerstaff, and V. Ben-Ezra
Responses of LDL and HDL particle size and distribution to omega-3 fatty acid supplementation and aerobic exercise
J Appl Physiol, September 1, 2009; 107(3): 794 - 800.
[Abstract] [Full Text] [PDF]


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J. Appl. Physiol.Home page
A. Cano, F. Ciaffoni, G. M. Safwat, P. Aspichueta, B. Ochoa, E. Bravo, and K. M. Botham
Hepatic VLDL assembly is disturbed in a rat model of nonalcoholic fatty liver disease: is there a role for dietary coenzyme Q?
J Appl Physiol, September 1, 2009; 107(3): 707 - 717.
[Abstract] [Full Text] [PDF]


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Am. J. Clin. Nutr.Home page
J. A Nettleton, J. F Polak, R. Tracy, G. L Burke, and D. R Jacobs Jr
Dietary patterns and incident cardiovascular disease in the Multi-Ethnic Study of Atherosclerosis
Am. J. Clinical Nutrition, September 1, 2009; 90(3): 647 - 654.
[Abstract] [Full Text] [PDF]


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Am. J. Clin. Nutr.Home page
C. Corvalan, R. Uauy, A. D Stein, J. Kain, and R. Martorell
Effect of growth on cardiometabolic status at 4 y of age
Am. J. Clinical Nutrition, September 1, 2009; 90(3): 547 - 555.
[Abstract] [Full Text] [PDF]


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J. Clin. Endocrinol. Metab.Home page
M. Brochu, M. F. Malita, V. Messier, E. Doucet, I. Strychar, J.-M. Lavoie, D. Prud'homme, and R. Rabasa-Lhoret
Resistance Training Does Not Contribute to Improving the Metabolic Profile after a 6-Month Weight Loss Program in Overweight and Obese Postmenopausal Women
J. Clin. Endocrinol. Metab., September 1, 2009; 94(9): 3226 - 3233.
[Abstract] [Full Text] [PDF]


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J. Clin. Endocrinol. Metab.Home page
D. Capalbo, A. Lo Vecchio, V. Farina, L. Spinelli, A. Palladino, C. Tiano, T. Lettiero, G. Lombardi, A. Colao, and M. Salerno
Subtle Alterations of Cardiac Performance in Children with Growth Hormone Deficiency: Results of a Two-Year Prospective, Case-Control Study
J. Clin. Endocrinol. Metab., September 1, 2009; 94(9): 3347 - 3355.
[Abstract] [Full Text] [PDF]


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NeurologyHome page
P. van Vliet, R.G.J. Westendorp, P. Eikelenboom, H. C. Comijs, M. Frolich, E. Bakker, W. van der Flier, and E. van Exel
Parental history of Alzheimer disease associated with lower plasma apolipoprotein E levels
Neurology, September 1, 2009; 73(9): 681 - 687.
[Abstract] [Full Text] [PDF]


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Hum ReprodHome page
M. Rizzo, K. Berneis, M. Hersberger, I. Pepe, G. Di Fede, G. B. Rini, G. A. Spinas, and E. Carmina
Milder forms of atherogenic dyslipidemia in ovulatory versus anovulatory polycystic ovary syndrome phenotype
Hum. Reprod., September 1, 2009; 24(9): 2286 - 2292.
[Abstract] [Full Text] [PDF]


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Clin. Chem.Home page
A. Baass, G. Dubuc, M. Tremblay, E. E. Delvin, J. O'Loughlin, E. Levy, J. Davignon, and M. Lambert
Plasma PCSK9 Is Associated with Age, Sex, and Multiple Metabolic Markers in a Population-Based Sample of Children and Adolescents
Clin. Chem., September 1, 2009; 55(9): 1637 - 1645.
[Abstract] [Full Text] [PDF]


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J. Nutr.Home page
E. A. Pipe, C. P. Gobert, S. E. Capes, G. A. Darlington, J. W. Lampe, and A. M. Duncan
Soy Protein Reduces Serum LDL Cholesterol and the LDL Cholesterol:HDL Cholesterol and Apolipoprotein B:Apolipoprotein A-I Ratios in Adults with Type 2 Diabetes
J. Nutr., September 1, 2009; 139(9): 1700 - 1706.
[Abstract] [Full Text] [PDF]


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Am J EpidemiolHome page
L. D. Bash, J. Coresh, A. Kottgen, R. S. Parekh, T. Fulop, Y. Wang, and B. C. Astor
Defining Incident Chronic Kidney Disease in the Research Setting: The ARIC Study
Am. J. Epidemiol., August 15, 2009; 170(4): 414 - 424.
[Abstract] [Full Text] [PDF]


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CirculationHome page
J. Yeboah, A. R. Folsom, G. L. Burke, C. Johnson, J. F. Polak, W. Post, J. A. Lima, J. R. Crouse, and D. M. Herrington
Predictive Value of Brachial Flow-Mediated Dilation for Incident Cardiovascular Events in a Population-Based Study: The Multi-Ethnic Study of Atherosclerosis
Circulation, August 11, 2009; 120(6): 502 - 509.
[Abstract] [Full Text] [PDF]


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Arch Intern MedHome page
H. Ward, P. N. Mitrou, R. Bowman, R. Luben, N. J. Wareham, K.-T. Khaw, and S. Bingham
APOE Genotype, Lipids, and Coronary Heart Disease Risk: A Prospective Population Study
Arch Intern Med, August 10, 2009; 169(15): 1424 - 1429.
[Abstract] [Full Text] [PDF]


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J. Lipid Res.Home page
K. L. Monda, C. M. Ballantyne, and K. E. North
Longitudinal impact of physical activity on lipid profiles in middle-aged adults: the Atherosclerosis Risk in Communities Study
J. Lipid Res., August 1, 2009; 50(8): 1685 - 1691.
[Abstract] [Full Text] [PDF]


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Am. J. Clin. Nutr.Home page
L. S Rallidis, J. Lekakis, A. Kolomvotsou, A. Zampelas, G. Vamvakou, S. Efstathiou, G. Dimitriadis, S. A Raptis, and D. T Kremastinos
Close adherence to a Mediterranean diet improves endothelial function in subjects with abdominal obesity
Am. J. Clinical Nutrition, August 1, 2009; 90(2): 263 - 268.
[Abstract] [Full Text] [PDF]


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Am. J. Clin. Nutr.Home page
L. Liu, J. A Nettleton, A. G Bertoni, D. A Bluemke, J. A Lima, and M. Szklo
Dietary pattern, the metabolic syndrome, and left ventricular mass and systolic function: the Multi-Ethnic Study of Atherosclerosis
Am. J. Clinical Nutrition, August 1, 2009; 90(2): 362 - 368.
[Abstract] [Full Text] [PDF]


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J. Clin. Endocrinol. Metab.Home page
S. Holewijn, M. den Heijer, D. W. Swinkels, A. F. H. Stalenhoef, and J. de Graaf
The Metabolic Syndrome and Its Traits as Risk Factors for Subclinical Atherosclerosis
J. Clin. Endocrinol. Metab., August 1, 2009; 94(8): 2893 - 2899.
[Abstract] [Full Text] [PDF]


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Arch Pediatr Adolesc MedHome page
J. M. Lee, A. Gebremariam, P. Card-Higginson, J. L. Shaw, J. W. Thompson, and M. M. Davis
Poor Performance of Body Mass Index as a Marker for Hypercholesterolemia in Children and Adolescents
Arch Pediatr Adolesc Med, August 1, 2009; 163(8): 716 - 723.
[Abstract] [Full Text] [PDF]


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Circ Cardiovasc GenetHome page
A. Q. Reuwer, M. T. Twickler, B. A. Hutten, F. W. Molema, N. J. Wareham, G. M. Dallinga-Thie, R. L. Bogorad, V. Goffin, M. Smink-Bol, J. J.P. Kastelein, et al.
Prolactin Levels and the Risk of Future Coronary Artery Disease in Apparently Healthy Men and Women
Circ Cardiovasc Genet, August 1, 2009; 2(4): 389 - 395.
[Abstract] [Full Text] [PDF]


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J. Am. Soc. Nephrol.Home page
I. H. de Boer, B. Kestenbaum, A. B. Shoben, E. D. Michos, M. J. Sarnak, and D. S. Siscovick
25-Hydroxyvitamin D Levels Inversely Associate with Risk for Developing Coronary Artery Calcification
J. Am. Soc. Nephrol., August 1, 2009; 20(8): 1805 - 1812.
[Abstract] [Full Text] [PDF]


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J. Nutr.Home page
C. P. Stewart, P. Christian, K. J. Schulze, S. C. LeClerq, K. P. West Jr, and S. K. Khatry
Antenatal Micronutrient Supplementation Reduces Metabolic Syndrome in 6- to 8-Year-Old Children in Rural Nepal
J. Nutr., August 1, 2009; 139(8): 1575 - 1581.
[Abstract] [Full Text] [PDF]


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CirculationHome page
J. Koskinen, M. Kahonen, J. S.A. Viikari, L. Taittonen, T. Laitinen, T. Ronnemaa, T. Lehtimaki, N. Hutri-Kahonen, M. Pietikainen, E. Jokinen, et al.
Conventional Cardiovascular Risk Factors and Metabolic Syndrome in Predicting Carotid Intima-Media Thickness Progression in Young Adults: The Cardiovascular Risk in Young Finns Study
Circulation, July 21, 2009; 120(3): 229 - 236.
[Abstract] [Full Text] [PDF]


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Eur J EndocrinolHome page
D. H St-Pierre, R. Rabasa-Lhoret, M.-E. Lavoie, A. D Karelis, I. Strychar, E. Doucet, and L. Coderre
Fiber intake predicts ghrelin levels in overweight and obese postmenopausal women
Eur. J. Endocrinol., July 1, 2009; 161(1): 65 - 72.
[Abstract] [Full Text] [PDF]


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J Am Med Inform AssocHome page
S. Shea, R. S Weinstock, J. A Teresi, W. Palmas, J. Starren, J. J Cimino, A. M Lai, L. Field, P. C Morin, R. Goland, et al.
A Randomized Trial Comparing Telemedicine Case Management with Usual Care in Older, Ethnically Diverse, Medically Underserved Patients with Diabetes Mellitus: 5 Year Results of the IDEATel Study
JAMIA, July 1, 2009; 16(4): 446 - 456.
[Abstract] [Full Text] [PDF]




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