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Clinical Chemistry 18: 509-515, 1972;
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Clinical Chemistry, Vol 18, 509-515, Copyright © 1972 by the American Association for Clinical Chemistry

Simple Automated Determination of Serum or Plasma Glucose by a Hexokinase/Glucose-6-Phosphate Dehydrogenase Method

William E. Neeley 1

1 Institute of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.

A simple, fast, efficient, and accurate automated method is described for determining the quantity of glucose in 50 µl of serum or plasma. A hexokinase/glucose-6-phosphate dehydrogenase reaction requiring incubation at room temperature is used. The method is specific for glucose, and is not affected by increased concentrations of endogenous chemical substances in uremic sera or by several common drugs that interfere with other glucose methods. Results of parallel assays, in which glucose oxidase and o-toluidine are used, are compared.


Key Words: glucose oxidase and o-toluidine methods compared • specificity for glucose • AutoAnalyzer • comparative interference by hemoglobin, bilirubin, drugs, lipemia, reducing sugars • normal range • incubation at room temperature

Submitted on December 29, 1971
Accepted on March 20, 1972




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Copyright © 1972 by the American Association for Clinical Chemistry.