Clinical Chemistry
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Clinical Chemistry 18: 516-518, 1972;
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Clinical Chemistry, Vol 18, 516-518, Copyright © 1972 by the American Association for Clinical Chemistry

Ultraviolet Spectrophotometric Measurement of Chlordiazepoxide in Plasma

Peter Jatlow 1

1 Department of Laboratory Medicine of Yale University School of Medicine, and the Clinical Chemistry Laboratory of the Yale—New Haven Hospital, New Haven, Conn. 06510.

A simple ultraviolet spectrophotometric method is described for measurement of chlordiazepoxide in blood. The drug is extracted into chloroform at pH 7.4 and, after a NaOH wash, extracted back into HCl. The drug is identified by its characteristic ultraviolet spectra at acidic and basic pH’s, and quantified from the absorbance value measured at the major acid peak of 247 nm. Although certain weakly basic drugs theoretically could interfere if present concurrently with chlordiazepoxide, we have not seen this during three years of experience. This procedure is easily combined with established methods for barbiturates and glutethimide. Specimens from more than 60 cases of documented chlordiazepoxide ingestion have had concentrations ranging from 0.2 to 6.6 mg/100 ml by this procedure.

Submitted on January 3, 1972
Accepted on March 23, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.