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Clinical Chemistry 18: 534-538, 1972;
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Clinical Chemistry, Vol 18, 534-538, Copyright © 1972 by the American Association for Clinical Chemistry

Characterization of Electrophoretic Lipoprotein Fractions: Immunochemical and Electron Microscopic Studies

Mario Werner 1 and Albert L. Jones 1

1 Department of Clinical Pathology and Laboratory Medicine, and Departments of Medicine and Anatomy, University of California, and the Veterans Administration Hospital, San Francisco, Calif.

Division of Laboratory Medicine, Barnes Hospital, St. Louis, Mo. 63110. Address reprint requests to this author.

To improve the characterization of electrophoretic lipoprotein subfractions, we developed two new techniques for analyzing lipoproteins after electrophoresis on thin agarose layers. Overlay with antisera exactly localizes specific apoproteins without any distortion caused by antigen diffusion; electron microscopy of eluted fractions determines the varying particle-size distribution. Applied together, these methods can detect individual differences between hyperlipemic samples that are not immediately apparent in the electrophoretic pattern, and should provide valuable new insight into the classification of hyperlipoproteinemias.


Key Words: antibody overlay technique • agarose gel electrophoresis • nephelometry • hyperlipoproteinemia phenotyping • protein localization on electropherograms

Submitted on March 9, 1972
Accepted on April 5, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.