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Clinical Chemistry 18: 563-564, 1972;
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Clinical Chemistry, Vol 18, 563-564, Copyright © 1972 by the American Association for Clinical Chemistry

Automated Determination of Serum Oxytocinase Activity

J. A. B. M. Peeters 1

1 Dept. of Clinical Chemistry (Head: P. J. Brombacher), De Wever-Ziekenhuis, Heerlen, The Netherlands.

An automated kinetic method is described for determination of oxytocinase activities in the sera of pregnant women. The reaction mixture consists of serum in phosphate buffer and the substrate L-cystine-bis-p-nitroanilide. The absorbance increase, due to the appearance of the reaction product, p-nitroaniline, is recorded during 2 min. With this method, 25 determinations per hour can easily be done.

Submitted on October 26, 1971
Accepted on February 25, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.