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Clinical Chemistry 18: 601-604, 1972;
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Clinical Chemistry, Vol 18, 601-604, Copyright © 1972 by the American Association for Clinical Chemistry

Simplified Serum Phosphorus Analyses by Continuous-Flow Ultraviolet Spectrophotometry

Elias Amador 1 and Jackie Urban 1

1 Division of Clinical Pathology, University Hospitals of Cleveland, and Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.

Serum phosphorus can be measured by continuous-flow uv spectrophotometry without reduction of the phosphomolybdate complex. The dilute sample is dialyzed into dilute (1 ml/100 ml) sulfuric acid, then mixed with an ammonium molybdate— sulfuric acid—"Tween 80" solution. The absorbance of the sample peaks is measured at 340 nm with a linear-absorbance spectrophotometer. Peak heights are directly proportional to concentration, because logarithmic conversion is performed within the spectrophotometer. The method obeys Beer’s law up to 10 mg of P per deciliter, and results correlate closely with those for standard methods based on reduced phosphomolybdate blue. The miniature manifold uses a 12-in. dialyzer. The sampling rate can be 60 to 120 samples per hour when a Gilford one-piece debubbler flow cell is used.


Key Words: AutoAnalyzer • linear absorbance spectrophotometry • nonreduction of phosphomolybdate complex

Submitted on February 22, 1972
Accepted on April 17, 1972




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