Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Clinical Chemistry 18: 601-604, 1972;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Amador, E.
Right arrow Articles by Urban, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Amador, E.
Right arrow Articles by Urban, J.

Clinical Chemistry, Vol 18, 601-604, Copyright © 1972 by the American Association for Clinical Chemistry

Simplified Serum Phosphorus Analyses by Continuous-Flow Ultraviolet Spectrophotometry

Elias Amador 1 and Jackie Urban 1

1 Division of Clinical Pathology, University Hospitals of Cleveland, and Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.

Serum phosphorus can be measured by continuous-flow uv spectrophotometry without reduction of the phosphomolybdate complex. The dilute sample is dialyzed into dilute (1 ml/100 ml) sulfuric acid, then mixed with an ammonium molybdate— sulfuric acid—"Tween 80" solution. The absorbance of the sample peaks is measured at 340 nm with a linear-absorbance spectrophotometer. Peak heights are directly proportional to concentration, because logarithmic conversion is performed within the spectrophotometer. The method obeys Beer’s law up to 10 mg of P per deciliter, and results correlate closely with those for standard methods based on reduced phosphomolybdate blue. The miniature manifold uses a 12-in. dialyzer. The sampling rate can be 60 to 120 samples per hour when a Gilford one-piece debubbler flow cell is used.


Key Words: AutoAnalyzer • linear absorbance spectrophotometry • nonreduction of phosphomolybdate complex

Submitted on February 22, 1972
Accepted on April 17, 1972




The following articles in journals at HighWire Press have cited this article:


Home page
Arch Otolaryngol Head Neck SurgHome page
A. Vissink, W. W. I. Kalk, K. Mansour, F. K. L. Spijkervet, H. Bootsma, J. L. N. Roodenburg, C. G. M. Kallenberg, and A. V. Nieuw Amerongen
Comparison of Lacrimal and Salivary Gland Involvement in Sjogren's Syndrome
Arch Otolaryngol Head Neck Surg, September 1, 2003; 129(9): 966 - 971.
[Abstract] [Full Text] [PDF]


Home page
Ann Rheum DisHome page
W W I Kalk, A Vissink, F K L Spijkervet, H Bootsma, C G M Kallenberg, and A V N. Amerongen
Sialometry and sialochemistry: diagnostic tools for Sjogren's syndrome
Ann Rheum Dis, December 1, 2001; 60(12): 1110 - 1116.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1972 by the American Association for Clinical Chemistry.