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Clinical Chemistry 18: 616-620, 1972;
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Clinical Chemistry, Vol 18, 616-620, Copyright © 1972 by the American Association for Clinical Chemistry

Automated Method for Screening Urine for Amphetamine and Some Related Primary Amines

E. R. Rutter 1

1 Home Office Central Research Establishment, Aldermaston, Nr. Reading, Berks, England.

This paper describes an automated method for extracting and detecting amphetamine and some related primary amines in urine. In the technique the color reaction given by primary amines with 2,4,6-trinitrobenzene sulfonic acid is used, and is sufficiently sensitive to detect the excretion of therapeutic doses of amphetamine. Samples of 4 ml of urine may be analyzed at a rate of 20 samples per hour, and the method is suitable for inclusion in a multi-channel drug-screening system.


Key Words: drug abuse • mass screening programs • interfering compounds • AutoAnalyzer

Submitted on March 6, 1972
Accepted on April 18, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.