Clinical Chemistry
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Clinical Chemistry 18: 647-653, 1972;
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Clinical Chemistry, Vol 18, 647-653, Copyright © 1972 by the American Association for Clinical Chemistry

Determination of Serum Albumin with the "SMA 12/60" by a Bromcresol Green Dye-Binding Method

James O. Westgard 1 and Michael A. Poquette 1

1 Department of Medicine and the Clinical Laboratories, University of Wisconsin Center for Health Sciences, Madison, Wis. 53706.

A bromcresol green (BCG) method for serum albumin has been adapted to the Technicon "SMA 12/60." Sensitivity and linearity critically depend on the concentrations of BCG and surfactant ("Brij-35"). With appropriate concentrations, linearity to 6.0 g/dl is achieved. Interaction is 2-3%. Standard deviations for quality control pools of 2.0 and 4.7 g/dl during three months of routine service were 0.06 and 0.11 g/dl. Values agree well with values determined by the Du Pont "ACA" (bias = 0.047 g/dl, SD = 0.191 g/dl, t = 1.746, r = 0.975, n = 53), by a modified Beckman "DSA" method in which BCG is also used (bias = 0.006, SD = 0.164, t = 0.398, r = 0.976, n = 115), and by electrophoresis on cellulose acetate (bias = 0.028, SD = 0.203, t = 1.006, r = 0.961, n = 54). Blank errors for hemolyzed samples (plasma hemoglobin up to 350 mg/dl), icteric samples (bilirubin up to 30 mg/dl), and lipemic samples (triglycerides up to 500 mg/dl) are 0.2 g/dl or less when the blank channel is not used. Normal range is estimated to be 3.5 to 4.8 g/dl (n = 145; 2.5 and 97.5 percentiles).


Key Words: normal range • comparison of results with Beckman DSA, Du Pont ACA, cellulose acetate electrophoresis • HABA dye method • interferences • precision • methodologic bias

Submitted on March 13, 1972
Accepted on May 3, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.